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Alternative Splicin...
Alternative Splicing of the Chromodomain Protein Morf4l1 Pre-mRNA Has Implications on Cell Differentiation in the Developing Chicken Retina
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- Boije, Henrik (författare)
- Uppsala universitet,Institutionen för neurovetenskap
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- Ring, Henrik (författare)
- Uppsala universitet,Medicinsk utvecklingsbiologi
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- Fard, Shahrzad Shirazi (författare)
- Uppsala universitet,Medicinsk utvecklingsbiologi
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- Grundberg, Ida (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Nilsson, Mats (författare)
- Stockholms universitet,Uppsala universitet,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Uppsala University
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- Hallbook, Finn (författare)
- Uppsala universitet,Medicinsk utvecklingsbiologi
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(creator_code:org_t)
- 2013-06-04
- 2013
- Engelska.
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Ingår i: Journal of Molecular Neuroscience. - : Springer Science and Business Media LLC. - 0895-8696 .- 1559-1166. ; 51:2, s. 615-628
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The proliferation, cell cycle exit and differentiation of progenitor cells are controlled by several different factors. The chromodomain protein mortality factor 4-like 1 (Morf4l1) has been ascribed a role in both proliferation and differentiation. Little attention has been given to the existence of alternative splice variants of the Morf4l1 mRNA, which encode two Morf41l isoforms: a short isoform (S-Morf4l1) with an intact chromodomain and a long isoform (L-Morf4l1) with an insertion in or in the vicinity of the chromodomain. The aim of this study was to investigate if this alternative splicing has a function during development. We analysed the temporal and spatial distribution of the two mRNAs and over-expressed both isoforms in the developing retina. The results showed that the S-Morf4l1 mRNA is developmentally regulated. Over-expression of S-Morf4l1 using a retrovirus vector produced a clear phenotype with an increase of early-born neurons: retinal ganglion cells, horizontal cells and cone photoreceptor cells. Over-expression of L-Morf4l1 did not produce any distinguishable phenotype. The over-expression of S-Morf4l1 but not L-Morf4l1 also increased apoptosis in the infected regions. Our results suggest that the two Morf4l1 isoforms have different functions during retinogenesis and that Morf4l1 functions are fine-tuned by developmentally regulated alternative splicing. The data also suggest that Morf4l1 contributes to the regulation of cell genesis in the retina.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Acetylation
- Avian
- Chromatin structure
- Development
- HAT
- HDAC
- Isoform
- Histon
- MRG15
- MRGX
- Neuron
- RCAS
- Retina
- Splicing
- Virus vector
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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